Figure 4.

Compared with traditional chemotherapy drug, 2ME2 has potent antileukemia activity. (A) AML cell lines were treated with increasing concentration of 2ME2 and cytarabine (0.5, 2.5, or 5μM) for 48 hours. Apoptosis was detected by annexin V-FITC/propidium iodide double staining. (B) Summary of the results of annexin V–FITC/7-AAD double staining from 3 independent experiments. **P < .01. (C) Cells were incubated with 2.5μM of 2ME2 or 5 μM cytarabine or in combination for 48 hours. The protein level of Bcl-xl and caspase 9 were then evaluated by Western blot.