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. 2016 Aug 18;11(8):e0160173. doi: 10.1371/journal.pone.0160173

Fig 2. Primer-probe sets do not produce signals with DNA template.

Fig 2

The primer-probe sets for the indicated transcripts were used in combined reverse-transcriptase-qPCR assays with HD11 cell line total RNA template (blue, 100, 10 and 1 ng), splenic DNA template (red, 20ng) or no template (green). The plots show background-subtracted fluorescence as a function of cycle (1–40) on a logarithmic scale. Missing points correspond to fluorescence levels lower than or equal to zero after automatic baseline subtraction. The heavy black line is the detection threshold used for all analyses.