Table 2. Primer and probe sequences for qRT-PCR.
| Gene | Type | Sequence | Exon | Amplicon* |
|---|---|---|---|---|
| ACTB | Forward | 5’-CAGGTCATCACCATTGGCAAT-3’ | 3 | 149/455 |
| Reverse | 5’-GCATACAGATCCTTACGGATATCCA-3’ | 4 | ||
| Probe | 5’-(FAM)CACAGGACTCCATACCCAAGAAAGATGGC(TAMRA)-3’ | 3/4 | ||
| B2M | Forward | 5’-AAGGAGCCGCAGGTCTAC-3’ | 2 | 151/1138 |
| Reverse | 5’-CTTGCTCTTTGCCGTCATAC-3’ | 4 | ||
| Probe | 5’(FAM)CCGGGATGAGCACGGTCTGAAGAATT(TAMRA)-3’ | 3/4 | ||
| HMBS | Forward | 5’-GGTTGAGATGCTCCGTGAGTTT-3’ | 3 | 153/798 |
| Reverse | 5’-GGCTCTTCTCCCCAATCTTAGAA-3’ | 4 | ||
| Probe | 5’-(FAM)CCTGACCTCTGCTTTGAGATTGTTGCCA(TAMRA)-3’ | 3/4 | ||
| HPRT1 | Forward | 5’-TGGTCAAAAGAACTCCTCGAAGT-3’ | 6 | 96/972 |
| Reverse | 5’-TGTAATCGAGGGCGTATCCAA-3’ | 7 | ||
| Probe | 5’-(FAM)TCCAACAAAGTCTGGCCGATATCCCA(TAMRA)-3’ | 6/7 | ||
| PGK1 | Forward | 5’-GTTTATGTCAATGATGCTTTTGGAA-3’ | 4 | 82/431 |
| Reverse | 5’-GCCTTTGCAAAATAATCCAGTTCT-3’ | 5 | ||
| Probe | 5’-(FAM)CATCGTGCTCACAGCTCCATGGTAGGT(TAMRA)-3’ | 4/5 | ||
| PLA2 | Forward | 5’-GCACAAGACATTTGGCAGTTGT-3’ | 2 | 138/5846 |
| Reverse | 5’-TGTGACATTTGTGGCTTTCCTTA-3’ | 3 | ||
| Probe | 5’-(FAM)CAACACATTGTGGTGGAACACCAGTACTCA(TAMRA)-3’ | 2/3 | ||
| PPIA | Forward | 5’-CCCGTCGTGTTCTTCGACAT-3’ | 1 | 140/>450 |
| Reverse | 5’-CCCTTGTAGCCAAATCCCTTCT-3’ | 2 | ||
| Probe | 5’-(FAM)CACCTTCGAGCTCTTCGCTGACAAGG(TAMRA)-3’ | 1/2 | ||
| RPL13 | Forward | 5’-TCGTGCTGGCAGAGGATTC-3’ | 3 | 71/908 |
| Reverse | 5’-TCGTCCGAGCAAACCTTTTG-3’ | 4 | ||
| Probe | 5’-(FAM)TAATGCCCGCCAGTTTAAGCTCTTCTAGGC(TAMRA)-3’ | 3/4 | ||
| RPLP0 | Forward | 5’-TTGGGCATCACCACAAAGATT-3’ | 4 | 82/431 |
| Reverse | 5’-CCCACTTTGTCTCCGGTCTTAA-3’ | 5 | ||
| Probe | 5’-(FAM)CATCACTCAGAATTTCAATGGTCCCTCGGG(TAMRA)-3’ | 4/5 | ||
| TBP | Forward | 5’-CTTCGTGCCCGAAATGCT-3’ | 4 | 82/1689 |
| Reverse | 5’-GCGCAGTAGTACGTGGTTCTCTT-3’ | 5 | ||
| Probe | 5’-(FAM)CTCATAATAACAGCAGCAAAACGCTTGGGA(TAMRA)-3’ | 4/5 |
* Lengths of predicted amplicons from mRNA/DNA in base pairs