Table I.
Antigen | Description of immunogen | Source, host species, catalog No., RRID |
Dilution |
---|---|---|---|
DBH (primary) | recombinant fragment corresponding to a region within amino acids 8 and 320 of Dopamine beta Hydroxylase |
Sigma, rabbit polyclonal, SAB2701977, RRID:AB_2566814 |
1:1000 |
GFP (primary) | Life Technologies, mouse monoclonal, A11120, RRID:AB_10055152 |
1:400 | |
Alexa Fluor 488- conjugated donkey anti- mouse IgG (H+L) |
Life Technologies, Donkey polyclonal, A21202, RRID:AB_10049285 |
1:400 | |
Anti-Mouse IgG (whole molecule)–Biotin antibody |
Sigma, goat polyclonal, B8774, RRID:AB_258613 |
1:400 | |
Texas, Red-conjugated Donkey Anti-Mouse IgG (H+L) |
Jackson ImmunoResearch, donkey polyclonal, RRID:AB_2566815 |
1:400 |