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. Author manuscript; available in PMC: 2017 Jan 11.
Published in final edited form as: Nat Chem Biol. 2016 Jul 11;12(9):709–716. doi: 10.1038/nchembio.2126

Figure 5. Functional effect of aptamers on β2AR–mediated Gαs and AC activation.

Figure 5

β2AR-dependent stimulation of AC activity and accumulation of cAMP was measured in HEK-293 membrane homogenates stably expressing β2AR (expression level: ~2.5 pmoles/mg) in the presence of 100 nM of isoproterenol (ISO) or combination of 100 nM ISO with aptamer (A1, A2, A13, A16 or control aptamer). Data represents the means ± s.e.m. of at least four independent experiments. Asterisks in the bar graphs denote significant differences (*P < 0.05; **P < 0.01) by one-way ANOVA (with Tukey’s multiple comparisons test) from results for AC activity performed with control aptamer.