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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Neuron. 2016 Jul 28;91(4):863–877. doi: 10.1016/j.neuron.2016.07.013

Figure 5. Ca2+ dynamics of tmc neurons in response to mechanical stimuli.

Figure 5

(A and B) Representative images showing the relative changes in Ca2+ levels in the SEZ with and without mechanical stimulation of the labellum. The Ca2+ dynamics were monitored using GCaMP6f. The color scale to the right of (H) shows ΔF/F.

(A) tmc-Gal4,UAS-GCaMP6f.

(B) tmc-Gal4,UAS-GCaMP6f;tmc1.

(C) The time course showing the Ca2+ dynamics in the SEZ of tmc-Gal4,UAS-GCaMP6f and tmc-Gal4,UAS-GCaMP6f;tmc1 flies. Shown are the fold changes in fluorescence intensity (ΔF/F0). The arrow indicates the onset of mechanical stimuli. n=5.

(D) The fold changes in peak fluorescence intensity (ΔF/F0) in response to different deflection distances for tmc-Gal4,UAS-GCaMP6f and tmc-Gal4,UAS-GCaMP6f;tmc1 flies. n=5.

The error bars indicate SEMs. *p<0.01. ANOVA tests with Scheffé’s post-hoc analysis.