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. Author manuscript; available in PMC: 2017 Aug 17.
Published in final edited form as: Neuron. 2016 Jul 28;91(4):863–877. doi: 10.1016/j.neuron.2016.07.013

Figure 6. Requirements for md-L neurons for sensing food hardness and viscosity.

Figure 6

(A and B) Intersectional genetic labeling of the central projections of md-L neurons.

(A) Central brain expression pattern of tmc-Gal4,UAS-mCD8::GFP.

(B) Central brain expression pattern of tmc-Gal4,UAS-mCD8::GFP;vGluT-Gal80.

(C) PER responses to foods containing 100 mM sucrose plus either 3% agarose or 1.5% HPC. Shown are the responses of controls and flies expressing the indicated transgenes. n=20.

(D and E) Images showing the soma and axon of an md-L neuron (tmc-Gal4,UAS-DsRed).

(D) Prior to laser treatment.

(E) After laser treatment.

(F) PER responses to foods containing 100 mM sucrose and either 3% agarose or 1.5% HPC before and after md-L neurons were exposed to laser treatments. n=15.

The error bars indicate SEMs. *p<0.05. ANOVA tests with Scheffé’s post-hoc analysis.