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. 2016 Apr 27;7(5):620–636. doi: 10.1002/wrna.1354

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© 2016 The Authors. WIREs RNA published by Wiley Periodicals, Inc.

This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Structural rearrangements of EF‐G on the ribosome during translocation. (a) EF‐G (blue in all structures) bound to the nonrotated pretranslocation ribosome containing A/A‐ and P/P‐ tRNAs93 (PDBID 4WPO). The N‐terminal domain of large subunit protein L9 covalently linked to the N‐terminus of EF‐G is shown as a transparent blue. (b) EF‐G bound to the fully rotated pretranslocation ribosome containing A/P*‐ and P/E‐ tRNAs55 (PDBID 4V7D); (c) EF‐G bound to partially rotated ribosomes containing chimeric ap/ap and pe/E‐ tRNAs42 (PDBID 4 W29) and (d) EF‐G bound to the nonrotated posttranslocation ribosome containing P/P‐ and E/E‐ tRNAs13 (PDBID 4V5F). 23S rRNA in all structures is shown in gray.