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Representative micrographs showing exocytic omega figures from the flash-and-freeze experiments. (A) C. elegans neuromuscular junctions, stimulated for 20 ms, and frozen at the end of the light pulse. Mouse hippocampal neurons, stimulated for 10 ms, frozen 5 ms after the end of the pulse using HPM 100 (B), and EM ICE (C). These structures are rarely observed in non-stimulated control or in specimens frozen at later time points after the stimulation.
