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Expression of TNF-α in vitro. (A) TNF-α expression of RAW246.7 cells determined by ELISA measurement 24/48 h after transfected with TNF-α shRNA by different polymers. (B) Relative levels of TNF-α mRNA expression was corrected by GAPDH. Data represent fold change of relative TNF-α expression normalized to GAPDH levels. (*p < 0.05, **p < 0.01).
