Figure 10.

Differential levels of C. burnetii rRNA species during growth. Levels of 16S rRNA and two fragments of 23S rRNA (23S F1 and 23S F2) were determined by Northern blot analyses. (A) Representative example of a Northern blot showing 23S F2, 16S rRNA, and 23S F1 performed on total RNA isolated from C. burnetii cultured for 1–5 d in ACCM-2 (In each case, 100 ng of total RNA and 0.05 mM probes were used). (B) Densitometric analysis of 16S rRNA (striped bars), 23S F1 (black bars), and 23S F2 (white, speckled bars). Values represent the means ± S.D. of the result of 3 independent Northern blot analyses from 2 different RNA preparations.