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. 2016 Aug 19;6:83. doi: 10.3389/fcimb.2016.00083

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Copyright © 2016 Warrier, Walter, Frangoulidis, Raghavan, Hicks and Minnick.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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In vitro coupled transcription-translation (IVTT) of C. burnetii's S23p. (A) 12.5% acrylamide [wt/vol] SDS-PAGE gel visualized by fluorescent scanning. A prominent ~13-kDa protein band (arrow head) was identified in the IVTT reaction using S23p+RBS (Lane 3) but was absent in IVTT reactions using S23p_439 (Lane 4) and S23p_493 (Lane 5). (B) Corresponding silver-stained 12.5% acrylamide [wt/vol] SDS-PAGE showing products of IVTT reactions involving S23p+RBS (Lane 3), S23p_439 (Lane 4) and S23p_493 (Lane 5) as a template. A positive (from kit, Lane 1) and no-template control (Lane 2) are also shown. Molecular weight values from protein standards are indicated to the left in kDa.