Figure 8.

Half-life of C. burnetii's IVS RNA in E. coli (IRW205). A representative RPA used to determine the in vivo IVS half-life is shown. (A) RPA blot with IVS band (480 bases) indicated by an arrowhead (3 μg RNA per lane and 800 pg of IVS probe were used). Lanes 1 and 2 show controls with biotin-labeled IVS probe in the absence or presence of RNase A/T1, respectively; Lanes 3–10 show RNA isolated at 5-min intervals from 0 to 35 min following rifampin treatment. (B) Densitometric analysis of IVS bands in blot lanes 3–10 vs. time of collection following rifampin treatment. The equation for the exponential curve and the R² value are inset and yield an IVS half-life of 3.9 min.