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. 2016 Jan 14;37(7):9077–9088. doi: 10.1007/s13277-015-4555-z

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© The Author(s) 2016

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — Expression of CDH12 in CRC cell lines. a CDH12 expression in CRC cell lines detected by qRT-PCR. b Upper panel effect of shCDH12 on CDH12 expression in SW620 cells compared with shNC which was transfected into scrambled shRNA and Mock which was transfected into Lipofectamine 2000. Lower panel ectopic expression of CDH12 in HCT116 transfected with lentivirus-CDH12. Vector and Blank groups were transfected into lentivirus NC and nothing, respectively. Column graph shows the relative grey level of each band compared with GAPDH