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. 2016 Aug 12;7:12433. doi: 10.1038/ncomms12433

Figure 3. Nod-factor-induced gene expression is promoted by MtDELLAs.

Figure 3

(a) The effect of GA3 on Nod-factor-induced gene expression was assayed using transgenic plants expressing pENOD11:GUS. Note: 6 h pre-treatment of GA3 suppresses expression of ENOD11 (right panel), but GA3 co-treatment with Nod factor does not suppress expression of ENOD11 (left panel). 1:mock, 2:10−4, 3:10−5, 4:10−6, 5:10−7 M GA3. Scale bars correspond to 1 cm. (b) Real-time PCR results revealed that GA3 suppresses expression of Nod-factor-induced genes. Treatment with 1 nM Nod factor for 6 h induced expression of ENOD11, VAPYRIN, NIN, ERN and RIP10 and the induction was reduced upon pre-treatment with 10−5 M GA3 for 6 h. (c–g) Real-time PCR results revealed that MtDELLAs required for induction of nodulin genes. Seven-day-old della2/della3 double mutants and R108 were treated with 1 nM Nod factor or mock (BNM medium) for 6 h. In wild type the nodulin genes were induced, however these genes could not be induced in della2/della3 double mutants after Nod factor treatment. These experiments were repeated three times with similar results, one representative experiment was shown. Error bars are standard error (n=3). The asterisk indicates a significant induction relative to control with Student's t-test (*P<0.01).