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. 2016 Aug 12;7:12433. doi: 10.1038/ncomms12433

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Copyright © 2016, The Author(s)

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(a) Yeast two-hybrid assays between NSP2 and MtDELLA1, MtDELLA2 or MtDELLA3. Yeast cells carrying different combinatory constructs are listed on the left. Serial dilutions (10 times) of yeast cells expressing the indicated proteins from the pDEST-GADT7(AD) and pDEST-GBKT7(BD) vectors were plated onto SD/-Leu-Trp(-LT) medium or SD/-Leu-Trp-Ade-His (-LTAH) medium with 30 or 60 mM 3-amino-1,2,4-triazole (3AT). (b) Pull-down assays between IPD3 and MtDELLA1, MtDELLA2 or MtDELLA3. MBP-IPD3 fusion protein but not MBP alone bound HIS-tagged MtDELLA1, MtDELLA2 or MtDELLA3. (c) Detection of protein–protein interactions in Arabidopsis protoplast by BiFC. YFP fluorescence of leaves co-transformed with MtDELLAs-nYFP and NSP2-cYFP. There is no protein interaction in the other three combinations: MtDELLAs-nYFP--cYFP, NSP2-cYFP--nYFP and cYFP--nYFP (Fig. 5c). Scale bars, 20 μm.