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. 2016 Aug 12;7:12467. doi: 10.1038/ncomms12467

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Copyright © 2016, The Author(s)

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(a–d) Cryo-CLEM. (a) Phase-contrast mode, (b,c) FM5-95 (red) staining, (d) electron microscopy. (e,f) HPF-FS electron microscopy, (e) cross-membrane without cell wall, (f) cross-membrane with a thick cell wall. Arrows indicate internal cross-membranes in the form of membrane vesicles and membrane arrays. Arrowheads indicate cross-membranes continuous with the extracellular membrane delimiting cellular segments. Scale bars: (a) 20 μm, (b) 5 μm, (c) 4 μm, (d) 500 nm, (e) and (f) 100 nm.