Skip to main content
. 2016 Aug 12;7:12529. doi: 10.1038/ncomms12529

Figure 1. PNI-induced increase in extracellular ATP within the spinal cord is dependent on VNUT.

Figure 1

(a) Schematic diagram of the experimental protocol for spinal ATP detection (Ipsi, ipsilateral; Contra, contralateral). (b) Measurement of extracellular ATP ([ATP]e) content in the ACSF media of ipsilateral and contralateral spinal cord slices taken from wild-type (WT) and VNUT-deficient (Slc17a9−/−) mice before (naive) and 7 days after PNI (naive: n=12, WT and Slc17a9−/−: n=16; *P<0.05, **P<0.01, one-way ANOVA with post hoc Tukey Multiple Comparison test). (c,d) Measurement of [ATP]e content in the ACSF media of ipsilateral and contralateral spinal cord slices taken from WT mice with or without (w/o) (c) tetanus toxin (TeNT) or (d) thapsigargin (TG) 7 days after PNI (c: n=6, d: n=11; *P<0.05, ***P<0.001, one-way ANOVA with post hoc Tukey Multiple Comparison test). Values are means±s.e.m.