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. 2016 Aug 12;7:12529. doi: 10.1038/ncomms12529

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Copyright © 2016, The Author(s)

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(a) Representative images showing immunofluorescence labelling of Iba1 (green) and CD11b (red) in the L4 spinal cord of Slc17a9^−/− and Slc17a9^+/+ mice 7 days after PNI (scale bar, 200 μm). (b) The intensity of CD11b immunofluorescence was quantified for the surface (lateral side of PKC-γ signals) and deep (medial side of PKC-γ signals) dorsal horn region of Slc17a9^−/− and Slc17a9^+/+ mice 7 days after PNI (n=4; **P<0.01, ***P<0.001, one-way ANOVA with post hoc Tukey Multiple Comparison test; scale bar, 100 μm). (c) Real-time PCR analysis of Aif1 (Iba1) mRNA using total RNA extracted from the spinal cord of Slc17a9^−/− and WT mice 7 days after PNI. Values represent the relative ratio of Aif1 mRNA (normalized to the value for 18S mRNA) to the contralateral side of WT mice (n=6; ***P<0.001, one-way ANOVA with post hoc Tukey Multiple Comparison test). Values are means±s.e.m. NS, not significant.