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. 2016 Aug 12;7:12529. doi: 10.1038/ncomms12529

Figure 5. Astrocytes, microglia and primary sensory neurons are not involved in VNUT-dependent neuropathic pain after PNI.

Figure 5

(a) PWT of astrocyte-specific VNUT conditional knockout (Gfap-Cre;Slc17a9fl/fl) mice and their Slc17a9fl/fl littermate controls before and after PNI (n=6; ***P<0.001 versus Contra (Slc17a9fl/fl), two-way ANOVA with post hoc Bonferroni test). (b) PWT of microglia-specific VNUT conditional knockout (Cx3cr1-CreERT2;Slc17a9fl/fl) mice and their Slc17a9fl/fl littermate controls before and after PNI. Both genotypes were injected with tamoxifen (TAM) 5 weeks before PNI. (Slc17a9fl/fl: n=6, Cx3cr1-CreERT2;Slc17a9fl/fl: n=5; **P<0.01, ***P<0.001 versus Contra (Slc17a9fl/fl +TAM), two-way ANOVA with post hoc Bonferroni test). (c) PWT of DRG neuron-specific VNUT conditional knockout (AAV-ESYN-Cre;Slc17a9fl/fl) mice and control AAV-ESYN-Venus; Slc17a9fl/fl mice before and after PNI (AAV-ESYN-Venus; Slc17a9fl/fl: n=8, AAV-ESYN-Cre;Slc17a9fl/fl: n=7; ***P<0.001 versus Contra (AAV-ESYN-Venus; Slc17a9fl/fl), two-way ANOVA with post hoc Bonferroni test). Values are means±s.e.m.