Figure 3.

Curcumin protected cholinergic neurons in mixed neuron/glia cultures. Mixed neuron/glia cultures were pre-treated with curcumin 10 μM, 1 h later, Aβ_1–42 25 μM was added to the mixed cultures. GW9662 1 μM was added into the cultures or cells were transfected with PPARγ siRNA 1 h before Aβ_1–42 treatment. (A) ELISA assay of ChAT. The results were obtained from six independent experiments. (B) LDH releasing to the culture medium. Data were expressed as mean ± SD with six individual experiments. ^**P < 0.01 vs. control cells, ^#P < 0.05, ^##P < 0.01 vs. Aβ_1–42-challenged cells, ^ΔP < 0.05, ^ΔΔP < 0.01 vs. curcumin treated cells.