Table 3.

The forward and reverse primer sequences used in the wild type 1 and 2, and the mutant type 1 and 2 insulin-like growth factor binding protein-3 3’UTR constructs

Primer name	Primer sequence
WT1 forward	5’-CAATGGTAAACTTGAGCATCTTTTCACTTTCCAGTAGT-3’
WT1 reverse	5’-CTAGACTACTGGAAAGTGAAAAGATGCTCAAGTTTACCATTGAGCT-3’
WT2 forward	5’-CGTCGAAGCGGCCGACCACTGACTTTGTGACTTT-3’
WT2 reverse	5’-CTAGAAAGTCACAAAGTCAGTGGTCGGCCGCTTCGACGAGCT-3’
MUT1 forward	5’-CAATGGTAAACTTGAGCATCTTTTCATCCAGTAGT3’
MUT1 reverse	5’-CTAGACTACTGGATGAAAAGATGCTCAAGTTTACCATTGAGCT-3’
MUT2 forward	5’-CGTCGAAGCGGCCGACCACTGACGTGACTTT-3’
MUT2 reverse	5’-CTAGAAAGTCACGTCAGTGGTCGGCCGCTTCGACGAGCT-3’

WT: Wild type; MUT: Mutant type.