Figure 3. Developmental myelination of the Mauthner axon in live nacre zebrafish.

(a) CARS images of the Mauthner axon on different days (image width: 8.4 μm). Representative images were selected from longitudinal imaging experiments (repeated imaging over time of same fish). (b) The myelin thickness and (c) the MM are plotted as a function of larval age. A steady increase in MM, but not in myelin thickness, was measured between 3 and 5 dpf. R² for linear regression and p-value for non-zero slope test are given in each graph.