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. 2016 Aug 18;16:188. doi: 10.1186/s12866-016-0810-8

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — PLNC8 αβ binds to P. gingivalis in a dose-dependent manner. Binding of P. gingivalis to PLNC8 αβ and to anti- P.gingivalis antibodies was analyzed by SPR. Both P. gingivalis ATCC 33277 (a) and W50 (b) were found to bind to immobilized PLNC8 αβ (280 nM). The binding was verified by pre-incubating the bacteria with different concentrations of soluble PLNC8 αβ prior to analysis, which resulted in a significantly reduced binding to the immobilized bacteriocins. Pre-incubation of P. gingivalis ATCC 33277 (c) and W50 (d) with increasing concentrations of soluble PLNC8 αβ prior to analysis reduced the bacterial binding to anti-P. gingivalis antibodies in a dose-dependent manner. Results are presented from three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001