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Improved strategies for isolating MEPs, MkPs, and ErPs in MPB and BM. (A) Improved strategy to enrich for MEPs (Lin−CD34+CD38midCD45RA−FLT3−MPL+CD36−CD41−), MkPs (Lin−CD34+CD38midFLT3−CD45RA−MPL+CD36−CD41+), and ErPs (Lin−CD34+CD38hiFLT3−CD45RA−MPL−). Dual Mk/E assay results for (B) MPB and (C) BM indicate similar enrichment levels in MPB and BM. Methylcellulose assay from the same populations in (D) MPB and (E) BM showing low contamination with other myeloid colonies (CFU-G/M/GM).
