Table 2.
Strains used in this work
| Strains | Description | Parent strain |
|---|---|---|
| Escherichia coli TOP10 | E. coli for electroporation hsdR, mcrA, lacZΔM15, recA | |
| CEN.PK111-61A | S. cerevisiae CEN.PK 111-61A MATα, ura3-52, his3-Δ1, leu2-3112, TRP1, MAL28c, SUC2 | |
| CEN.PK113-1A | S. cerevisiae CEN.PK 113-1A MATα, URA3, HIS3, LEU2, TRP1, MAL28c, SUC2 | |
| S. cerevisiae ATCC 90845 | MATα, his3Δ 200, ura3-52, leu2 Δ 1, lys2-Δ 202, trp1-Δ63 | |
| A. niger ATCC 1015 | Template DNA for gene gaaC | |
| Schizosaccharomyces pombe | Template DNA for gene his5 | |
| H4362 | S. cerevisiae CEN.PK 111-61A+ gaaC (MATα, ura3-52, HIS5, leu2-3112, TRP1, MAL28c, SUC2) | S. cerevisiae CEN.PK 111-61A |
| H4410 | S. cerevisiae CEN.PK 111-61A + gaaC + gaaD (MATα, ura3-52, HIS5, LEU2, TRP1, MAL28c, SUC2) | H4362 |
| H4425 | S. cerevisiae CEN.PK111-61A + gaaC + gaaD + gaaA (MATα, URA3, HIS5, LEU2, TRP1, MAL28c, SUC2) | H4410 |
| H4531 | S. cerevisiae CEN.PK111-61A + gaaC + gaaD + gaaA + lgd1 (MATα, URA3, HIS5, LEU2, TRP1, MAL28c, SUC2, can1−) | H4425 |
| H4535 | S. cerevisiae CEN.PK111-61A + gaaC + gaaD + gaaA + lgd1 + gat1-gfp (MATα, URA3, HIS5, LEU2, TRP1, MAL28c, SUC2, can1− , HO −) | H4531 |