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. 2016 Aug 19;6:31764. doi: 10.1038/srep31764

Figure 2. Real-time quantitative RT-PCR analysis of OsGas2 expression profiles in rice suspension cells under different stresses.

Figure 2

(a) DTT induced the expression of OsbZIP50 in wild type and OsGas2 RNAi cell lines. Protoplasts were prepared from suspension cells, and treated with 6.4 mM DTT for 18 h at 26–28 °C. (b) OsGAS2 expression in the protoplasts from suspension cells with the treatment of 6.4 mM DTT for different times. (c–e) OsGAS2 expression in 8-day-old seedling roots with the treatment of 4 °C (c), 150 mM NaCl (d), and 40% PEG 6000 (e) for different times. Total RNA was isolated for RT-PCR, and experiments were repeated three times.