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. 2016 Aug 19;6:31764. doi: 10.1038/srep31764

Figure 3. Subcellular localization of Grp94, GAS2 and TPR-containing HSP40 in protoplasts.

Figure 3

Constructs carrying 35S:Grp94–GFP, 35S:GAS2–GFP, and 35S:GFA2–GFP, were introduced respectively into protoplasts from the stems of rice seedlings by PEG-calcium-mediated transformation. GFP fluorescence were observed after 16-h incubation with a PerkinElmer UltraVIEW VoX confocal microscope. ER-Tracker™ Blue-White DPX (E12353) was used as an ER localization control. Experiments were repeated at least five times. Bar = 5 μm.