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. 2016 Aug 19;6:31764. doi: 10.1038/srep31764

Figure 6. Cleavage product of Gas2 amplified caspase-3-like activity in mesophyll protoplasts.

Figure 6

(a) RT-PCR analysis of N-terminal fragment (N) and C-terminal fragment (C) expressions in mesophyll protoplasts. Total RNA was prepared from protoplasts with or without (control) treatment of Dex for 18 hour. 17S RNA was used as a loading control. (b) Caspase-3 like activity in mesophyll protoplasts under the expression of N-terminal fragment (N) and C-terminal fragment (C). Expression of N and C were induced by Dex for 18 hours, and then the protoplasts were treated at 48 °C for 15 min and allowed to recover at 28 °C for 6 h. The protoplast with empty conditional expression vector was used as control. An equal amount of cytosolic fractions were incubated with Ac-DEVD-AMC, and the AMC fluorescence was measured within 60 min by a Microplatereader at 30 °C. RFU/mg: relative fluorescence units per mg protein.