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. 2016 Aug 19;6:29176. doi: 10.1038/srep29176

Figure 3. Expression of EZH2 influenced SOX9 expression in normal chondrocytes.

Figure 3

(A) Normal chondrocytes (P2) were infected with Lenti-EZH2. A significant increase of EZH2 and a obvious decrease of SOX9 were demonstrated by Western-blotting. (B,C) A quantitative analysis of EZH2 and SOX9 protein expression confirmed the Western-blotting results. (D) The normal chondrocytes (P2) were treated with IL-1β (10 ng/ml) for 24 hours to induce chondrocyte pathological change followed by knockdown by siEZH2 or treatment by pharmacological inhibitor EPZ005687. The effect of each treatment was investigated by Western-blotting and quantitative analysis (E,F). IL-1β treatment notably enhanced EZH2 expression and inhibited SOX9 expression. Subsequently, knockdown of EZH2 by siEZH2 downregulated EZH2 expression and ameliorate the inhibition of SOX9. Meanwhile, the inhibition of SOX9 was also attenuated following treatment with the EZH2 inhibitor EPZ005687 (5.6 μmol/L). Values are means ± SD (n = 3). *p < 0.05, **p < 0.01.