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. 2016 Aug 19;6:31615. doi: 10.1038/srep31615

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Copyright © 2016, The Author(s)

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(a) SGO1 knockdown inhibited cell proliferation only in MYCN-overexpressing SH-EP cells. The number of the cells was counted 6 days after non-target or SGO1-targeted shRNA lentivirus infection. Upper panel, relative viability; lower panel, SGO1 knockdown efficiency. Data are expressed as the mean ± SE of at least three independent experiments. (b) SGO1 knockdown inhibited cell proliferation only in MYCN-amplified neuroblastoma cell lines. Number of cells counted at 4, 5, 6, 7, or 8 days after non-target or SGO1-targeted shRNA lentivirus infection. Upper panel, relative viability; bottom panel, SGO1 knockdown efficiency. (c) Flow cytometry profiles of control and SGO1-knockdown cells at 48 hrs after non-target or SGO1-targeted shRNA lentivirus infection. Lower panel shows SGO1 knockdown efficiency.