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. 2016 Aug 19;6:31774. doi: 10.1038/srep31774

Figure 3. Effect of cargo size and exportin Crm1 mutation on the limits of NES-GFPs.

Figure 3

(A,B) Localization of monomeric GFPs with NLS and NES (A) and 3×GFP with NLS and NES (B). Cells expressing indicated modified GFPs from PYK1pro on gTOW plasmids cultured in –Ura medium were observed. Arrowheads indicate the positions of nuclei. C) Copy numbers of gTOW plasmids containing indicated modified GFPs expressed from PYK1pro under –Leu–Ura conditions. D) Copy numbers of gTOW plasmids containing indicated modified GFPs expressed from indicated promoters within the wild type (CRM1) and the crm1-1 mutant cells cultured at 30 °C in –Ura conditions. Error bars represent standard deviations.