Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 19;6:31752. doi: 10.1038/srep31752

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) A schematic representation of centromere 1 and the position of a inserted marker gene (otr1R::ura4). The pericentromeric heterochromatin region is indicated. (b) A fivefold serial dilution assay to examine the silencing of otr1R::ura4. Wild-type (WT) cells with silenced ura4⁺ grow normally on medium containing 5′-FOA, while loss of silencing kills cells on 5′-FOA. (c) RT-PCR analysis of otr1R::ura4⁺ and pericentromeric repeat (dh) RNA levels relative to a control act1⁺. The relative level in WT cells was arbitrarily designated as 1. Each column shown in (c) and below represents the mean ± s.d. from three biological repeats. (d,e) ChIP analysis of RNA Polymerase II (d) and H3K9me2 (e) at otr1R::ura4 and dh relative to fbp1⁺. Relative enrichment in WT cells was arbitrarily designated as 1. (f) RT-PCR analysis of tlh1⁺ and cenH RNA levels relative to a control act1⁺. (g) ChIP analysis of H3K9me2 at tlh1⁺ and cenH relative to fbp1⁺.