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. 2016 Aug 19;6:31675. doi: 10.1038/srep31675

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Copyright © 2016, The Author(s)

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(A,C) Cells were treated with vehicle control (Ctrl), 10 IU/mL hCG (A) or 1 mM 8-Br-cAMP (C) for various periods of time, and the mRNA levels of SPRY2 were examined by RT-qPCR. The level of SPRY2 mRNA at each time point was normalized to that of GAPDH mRNA at the same time point. (B,D) Cells were treated with vehicle control (Ctrl), 10 IU/mL hCG (B) or 1 mM 8-Br-cAMP (D) for various periods of time, and the protein levels of SPRY2 were examined by western blot. The results are expressed as the mean ± SEM of at least three independent experiments. Values without a common letter are significantly different (p < 0.05).