Figure 5.
Autophagy induced by overexpression of the BH3-only protein BCL2L11 is strictly dependent on BAX and BAK1. (A) Overexpression of BCL2L11s only induces LC3B lipidation when BAX and BAK1 are present. MEFs bearing a doxycycline-inducible BCL2L11s lentiviral construct were treated with doxycycline for the indicated times and analyzed by western blotting. (B) Western blot of wild-type and bax−/−bak1−/− MEFS after overexpression of BCL2L11s with doxycycline for 4 h with or without 10 μM treatment with the autolysosome inhibitor chloroquine (CQ). The lower ACTB panels are of the same samples, diluted, and run on a separate gel.