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. 2016 Aug 18;17:119. doi: 10.1186/s12863-016-0427-9

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Development of and results observed for the qPCR marker analysis based on scaffold_13. a Scheme for primer design over a specific genomic region on scaffold_13 of 0B, 1B and 2B samples. Note the higher coverage for 1B (5,000× coverage) and 2B (10,000× coverage) samples compared with the 0B sample. b Graphic plot depicting the relative number of copies detected among B+ samples (1B and 2B) and control samples (0B). Note that 2B samples (asterisks) present approximately two-fold as many copies compared with the 1B samples. The standard deviation is presented for each sample. c FISH using the qPCR marker region sequence as a probe. An arrow indicates the B chromosome, and the scale bar is 5 μm