Figure 1.

Western blot confirming binding of mAbs to CPS. B. pseudomallei (Bp) 1026b, B. mallei (Bm) China 7, CPS purified from Bp RR2683, or B. thailandensis (Bt) E264 were incubated with proteinase-K, separated by SDS PAGE, and subsequently transferred to a nitrocellulose membrane. Membranes were probed with 0.5 µg of mAb and binding was detected with an HRP-conjugated goat anti-mouse kappa chain antibody. IgG₃ mAb 3C5 and IgG₁ mAb 2A5 bind to a high molecular weight, proteinase-K resistant antigen found in B. pseudomallei, B. mallei, and purified CPS. No mAb binding was visualized against B. thailandensis, which lacks CPS.