Figure 2.

Binding specificity of subclass-switch family mAbs and 3C5 IgG₃ F(ab')₂ fragments given as a function of response units (RU) generated over time. Data are shown from a representative experiment. A BIAcore X100 instrument was used to determine the affinity of each mAb for CPS. CPS purified from Bp RR2683 was conjugated to biotin and immobilized on a SA sensor chip. Binding was analyzed by injecting 8 samples diluted 2-fold (dilutions; 0.33–333 or 12–3,333 nM) for 60 s, followed by 120 s of passive dissociation.