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. 2016 Apr 20;7(6):691–701. doi: 10.1080/21505594.2016.1176655

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Figure 4. — Comparison of binding activity within each mAb subclass by Western blot. B. pseudomallei Bp82 total cell lysate (87 μg) was separated by SDS-PAGE and transferred onto nitrocellulose membrane. The membranes were probed with mAb 3C5 family (panel A) and 2A5 family (panel B) using a miniblotter. 3C5 IgG3 shows substantially higher reactivity to CPS compared to the other subclasses. The reactivity between different subclasses of mAb 2A5 and CPS was comparable. 2A5 and 3C5 antibody concentration was prepared from 1mg/ml stock. Exposure time for Western blots in panel A and B were different.