Figure 1.
Maximum parsimony (MP) tree showing the phylogenetic relationship of cultured Leptotrichiaceae species and those only represented by environmental 16S rRNA gene sequences. The tree was calculated in ARB using DNAPARS and based on 16S rRNA gene sequences spanning at least gene termini 97 to 1356.138 Shorter sequences were added after tree construction without changing the overall tree topologies. Large circles represent nodes that were at least also present with high bootstrap support in the Maximum likelihood (ML) tree. Small circles mark nodes that were also present in the MP and neighbor joining tree, but in the ML tree only supported by bootstrap values <70%. GenBank/EMBL/DDBJ accession numbers of the sequences are given in parentheses. Numbers at branch nodes refer to bootstrap values >70% (100 replicates). Fusobacterium nucleatum subsp. nucleatum ATCC 25586T was used as outgroup. Bar: 0.1 nucleotide substitutions per nucleotide position. T marks type strain sequences.