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Effect of various extraction buffers on the EPR signal after incubating Arabidopsis seedlings with 4-hydroxy-TEMPO radical. Arabidopsis seedlings (2 week old) were incubated with 500 µM 4-hydroxy-TEMPO radical for 30 min, washed thoroughly with DDW, and then flash frozen. For extraction, Ethanol (75%) was supplemented with: DDW, 10 mM EDTA, 20 mM N-ethylmaleimide, or a combination of EDTA and NEM as indicated.
