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. 2016 Feb 3;7(15):19468–19482. doi: 10.18632/oncotarget.7153

Figure 7. Dax-1 is a target gene of FXRα.

Figure 7

A. Testicular mRNA expression of Shp and Dax-1 normalized to b-actin levels in whole testis of C57Bl/6J mice treated with vehicle or GW4064 for 5 days (n=5 to 10 per group). B. mRNA expression of Fxrα, Lhcgr, Fshr and Oct3/4 normalized to β-actin levels in interstitial and tubular compartment of 15-days-old testis of C57Bl/6J. C. mRNA expression of Dax-1 normalized to β-actin levels in MA-10 cells treated for 6hours or 12 hours with vehicle or GW4064 (n=10 per group). D. Sequences of FXRE putative biding site (IR1) in human and mouse DAX-1 promoters. E. CV1cells were transfected with pCMX-mFXRα, pCMX-mRXRα, or both receptor plasmids in the presence of the 1.2kbDAX-1 promoter (hDAX1) linked to luciferase. Cells were treated with GW4064 (1 μM). F. Testicular mRNA expression of Shp and Dax-1 normalized to β-actin levels in whole testis of Fxrα−/− mice treated with vehicle or GW4064 for 5 days (n=5 to 10 per group). G. Relative progesterone levels in medium of MA10 cells transfected with siRNA-control or siRNA directed against Dax-1 and treated for 12 hours with vehicle or GW4064 (n=6-15 per group). H. mRNA expression of Star normalized to β-actin levels in MA10 cells transfected with siRNA-control or siRNA directed against Dax-1 and treated for 12 hours with vehicle or GW4064 (n=10 to 15 per group). In all of the panels data are expressed as the means ± SEM. Statistical analysis:*, p<0.05; **, p<0.01, ***, p<0.005 vs.respective control group.