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. 2016 Jan 9;7(15):19483–19498. doi: 10.18632/oncotarget.6869

Figure 5. Knockdown of PCBP2 inhibits glioma cell migration and invasion.

Figure 5

A. Western blotting of PCBP2 expression in 4 glioma cell lines (T98G, U87 MG, A172, U251) transfected with NC siRNA and PCBP2 siRNA for 48 h. (B., C.) Transwell migration assays of 4 glioma cell lines (T98G, U87 MG, A172, U251) were performed after transfection with NC siRNA and PCBP2 siRNA for 48-72 h. The results are representative of at least three independent experiments. Graphs indicate the average number of cells per field of the indicated cell lines in migration assays. Data show the mean ± SD, *P<0.05,**P<0.01. (D., E.) Invasion assays of 2 glioma cell lines (T98G, U87 MG) were performed after transfection with NC siRNA and PCBP2 siRNA for 48-72 h. The results are representative of at least three independent experiments. Graphs indicate the average number of cells per field of the indicated cell lines in invasion assays. (F., G.) Wound-healing assays in T98G cells were performed after transfection with NC siRNA and PCBP2 siRNA for 0-96 h. Pictures were taken every 24 h. Graph represents the width of the remaining open wound calculated in relation to time 0. H. Co-knockdown of ARHGDIA and PCBP2 for a rescue study. Western blotting analysis revealed that ARHGDIA siRNA and PCBP2 siRNA inhibit both of their targeted proteins after transfection with ARHGDIA siRNA and PCBP2 siRNA individually or co-transfection into T98G cells. (I., J.) The transwell assays were performed after transfection with ARHGDIA siRNA and PCBP2 siRNA individually or co-transfection into T98G cells. Graphs indicate the average number of cells per field of the indicated cell lines in migration assays. Data show the mean ± SD, *P<0.05,**P<0.01.