Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
a. The seed sequence of miR-1296 is complementary to the 3′UTR of CCND1. b.-c. Luciferase assay showing reduction in reporter activity (relative luciferase units) after co-transfection of CCND1-3′UTR with miR-1296 in 436 and 231 cells, respectively. The mutant 3′UTR had no effect on reporter activity. d.-e. Western blot analysis showing suppression of CCND1 protein levels in 436 and 231 cells after miR-1296 overexpression, respectively. *p < 0.05.
