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. 2016 Feb 22;7(15):19693–19708. doi: 10.18632/oncotarget.7564

Figure 1. HDAC9 is overexpressed in the most aggressive breast cancer cells.

Figure 1

A. Total HDAC9 mRNA levels were measured in fourteen breast tumor cell lines classified as luminal (n=7), basal A (n=2) and basal B (n=5). Results are expressed relative to the HDAC mRNA levels of the MCF7 cells and represent mean ± SD of 3 independent cell cultures. B. Proteins were extracted from luminal (n=4), basal A (n=2) and basal B (n=4) breast tumor cells and analyzed by western-blot using anti-HDAC9 antibody. Actin was used as a loading control. This western-blot is representative of two independent experiments. C. Total HDAC9 mRNA levels were measured in the MCF10 mammary cell lines. Results are expressed relative to the HDAC mRNA levels of the MCF10A cells and represent mean ± SD of 3 independent cell cultures.