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. 2016 Feb 25;7(15):19723–19737. doi: 10.18632/oncotarget.7736

Figure 4. miR-137 and miR-6500-3p target CENPE, KIF14 or NCAPG, which are upregulated in pHGGs.

Figure 4

Venn diagram showing overlapping mRNAs based on microarray data (top 50 upregulated mRNAs in pHGGs) and bioinformatics prediction results (Targetscan or SVmicro). The bioinformatic analysis indicates the miR-137 A. and miR-6500-3p B. targets. RT-qPCR and IHC analyses confirmed higher mRNA and protein levels of CENPE C., KIF14 D. and NCAPG E. in pHGGs (mRNA: n = 12; protein: n = 3) than in pLGGs (mRNA: n = 12; protein: n = 3). RT-qPCR results are presented as mean±SD for duplicate samples. **p < 0.01 by t-test. Scatter plots illustrating the negative correlations between miR-137 and CENPE F., miR-6500-3p and KIF14 G., and miR-6500-3p and NCAPG H. in pediatric gliomas.