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. 2016 Mar 2;7(15):19794–19812. doi: 10.18632/oncotarget.7847

Figure 4. DDA1 facilitates colon cancer cell proliferation and cell cycle progression in vitro and in vivo.

Figure 4

(A) DDA1 expression was assessed using western blotting in eight colon cancer cell lines and two normal colon epithelial cell lines. (B) Overexpression or knockdown of DDA1 accelerated or inhibited the proliferation of colon cancer cells, respectively. (C) HT-29 and SW480 cell lines were subcutaneously injected into nude mice; tumor weight and volume growth curves are shown at three weeks post-injection (n = 5, *P < 0.05). (D) FACS analyses of cells in every phase of the cell cycle after transfection with LV-DDA1 or DDA1 shRNA#1 in HT-29 and SW480 cells (*P < 0.05). (E) Detection of cell cycle-related proteins in DDA1 overexpressed and knocked down cell lines. (F) IHC staining of DDA1, Ki-67, c-myc, and CyclinD1 in DDA1 overexpressing and control xenografts (magnification ×200).