Figure 2. RASSF6 expression in colon tissues and CRC cell lines.

(A) Immunohistochemical staining of RASSF6 expression in tissue microarray. Weak or negative staining for RASSF6 was detected in adenocarcinoma. (B) Adjacent benign glands were showing strong RASSF6 staining. (A and B) are representative images from the same patient. (C) Comparison of staining score between 52 cases of adenocarcinoma and adjacent normal tissue. Staining score is classified as weak or negative (0), mild (1), moderate (2), and strong (3) (***P < 0.001, paired t test). (D) Transcriptional level of RASSF6 in colorectal cancer cell lines (SW480, LoVo, and HT-29) was analyzed using quantitative real time-PCR. RASSF6 was expressed in all cell lines, relatively high in HT-29 cells and low in LoVo cells, with GAPDH as the normalized reference gene. (E) Western blot analysis of RASSF6 protein levels that normalized to GAPDH expression. The result is consistent with transcriptional level analysis. All data are shown as mean ± SE.