Figure 3. Effect of ZSTK474 on expression or phosphorylation of the cell cycle-related proteins in MCF-7 cells.

The cells were treated with 0, 0.1, 0.5, 2, 4 μM of ZSTK474 for 24 h. After treatment, the lysates of whole cell or nucleus were prepared by using the respective lysis buffer, to be available for western blot. The blots were exposed to anti- cyclin D1, p-GSK-3β, p27, phosphorylated p-Rb, β-actin (for whole cell) or Lamin B (for nucleus). Signals of the respective proteins in whole cell A. or nucleus B. after treatment with ZSTK474 were shown. Experiments were performed independently for three times.