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. 2016 Mar 3;7(15):19910–19927. doi: 10.18632/oncotarget.7889

Figure 2. AGP-activated medium exhibits tumor-selective cytotoxicity.

Figure 2

(A) Different volumes of DMEM (1–5 ml) were irradiated with AGP. A375 cells were cultured in each AGP-activated medium for 24 or 72 h, and measured for their cell growth (absorbance at 450 nm) using a cell proliferation assay kit. Control medium was prepared by exposure to helium gas without discharge, and used for cell culture. The data represent means ± SEM of three independent experiments (B) Representative phase-contrast images of cells cultured in non-treated (NT) (left), control (middle), or AGP-activated (right) medium for 72 h. Bars = 500 μm. (C) A375 cells were cultured in NT, control, or AGP-activated medium for 24, 48, and 72 h, and measured for their cell growth (absorbance at 450 nm). (D) Cancer cells (A375, A549, MG63) and non-transformed cells (HDFs, melanocytes) were cultured in control or AGP-activated medium for 72 h, and measured for their cell viability using the WST-8 assay. The data shown are percentages of the value in control cells set at 100, and represent means ± SEM of three independent experiments. ***P < 0.001.